non-targeting scrambled sirna Search Results


stabletm sirna oligo nontargeting scrambled sirna  (Shanghai GenePharma)
90
Shanghai GenePharma stabletm sirna oligo nontargeting scrambled sirna
GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled <t>siRNA</t> (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.
Stabletm Sirna Oligo Nontargeting Scrambled Sirna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non-targeting+scrambled+sirna/stabletm+sirna+oligo+nontargeting+scrambled+sirna/pmc06838379-140-10-5
Average 90 stars, based on 1 article reviews
stabletm sirna oligo nontargeting scrambled sirna - by Bioz Stars, 2026-09
90/100 stars
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scrambled sirnas  (Shanghai GenePharma)
90
Shanghai GenePharma scrambled sirnas
GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled <t>siRNA</t> (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.
Scrambled Sirnas, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non-targeting+scrambled+sirna/scrambled+nontargeting+sirna/pmc06797509-97-7-22
Average 90 stars, based on 1 article reviews
scrambled sirnas - by Bioz Stars, 2026-09
90/100 stars
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and scrambled non-targeting sirna sense, 5′-uucuccgaacgugucacgutt-3′ and anti-sense, 5′-acgugacacguucggagaatt-3′  (Shanghai GenePharma)
90
Shanghai GenePharma and scrambled non-targeting sirna sense, 5′-uucuccgaacgugucacgutt-3′ and anti-sense, 5′-acgugacacguucggagaatt-3′
GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled <t>siRNA</t> (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.
And Scrambled Non Targeting Sirna Sense, 5′ Uucuccgaacgugucacgutt 3′ And Anti Sense, 5′ Acgugacacguucggagaatt 3′, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non-targeting+scrambled+sirna/and+scrambled+non+targeting+sirna+sense++5++uucuccgaacgugucacgutt+3++and+anti+sense++5++acgugacacguucggagaatt+3+/pmc04812116-105-25-26
Average 90 stars, based on 1 article reviews
and scrambled non-targeting sirna sense, 5′-uucuccgaacgugucacgutt-3′ and anti-sense, 5′-acgugacacguucggagaatt-3′ - by Bioz Stars, 2026-09
90/100 stars
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non-targeting sirna negative control (scrambled  (Sigma-Genosys)
90
Sigma-Genosys non-targeting sirna negative control (scrambled
GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled <t>siRNA</t> (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.
Non Targeting Sirna Negative Control (Scrambled, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/non-targeting+scrambled+sirna/non+targeting+sirna+negative+control++scrambled/pm23775590-79-1-8
Average 90 stars, based on 1 article reviews
non-targeting sirna negative control (scrambled - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled siRNA (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.

Journal: EBioMedicine

Article Title: Variants in oxidative stress-related genes affect the chemosensitivity through Nrf2-mediated signaling pathway in biliary tract cancer

doi: 10.1016/j.ebiom.2019.08.037

Figure Lengend Snippet: GPX4, CAT, and GSR regulate Nrf2 protein expression through ROS. (a and b) Representative FACS profile of ROS levels, which was measured by H2DCFDA staining, in GBC-SD (a) and QBC-939 (b) when GPX4, CAT, or GSR was overexpressed or knockdown. (c and d) Analysis of correlation between the protein level of Nrf2 and the mRNA level of GPX4 (c, left) or GSR (c, right) was performed in 36 GBC tissues, and the mRNA level of CAT (d, left) or GSR (d, right) was performed in 52 CC tissues. Spearman's correlation was used. (e and f) Immunoblot and qPCR analysis of Nrf2, and it target NQO1 from GBC-SD transfected with scrambled siRNA (si-Con) or siRNA (si- GPX4 and si- GSR ) and empty vector, GPX4 , or GSR (e), and from QBC-939 transfected with si-Con or siRNA (si- CAT and si- GSR ) and empty vector or CAT or GSR (F). n = 3; Bar, SEM. (g and i) qPCR analysis of NFE2L2, NQO1 and ABCG2 expression in GBC-SD (g: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (i: si-Con, si- CAT and si- GSR ) cells cultured in standard media supplemented with or without 10 mM NAC. n = 3; Bar, SEM. (h and j) Immunoblots of Nrf2, NQO1 and ABCG2 protein in GBC-SD (h: si-Con, si- GPX4 and si- GSR ), and in QBC-939 (j: si-Con, si- CAT and si- GSR ) cells incubated with or without 10 mM NAC. * P < .05, ** P < .01, *** P < .001, Student's t- test.

Article Snippet: The following StableTM siRNA oligo (Genepharma, Shanghai, China) were used: nontargeting scrambled siRNA, human GPX4 siRNA, human CAT siRNA, human GSR siRNA, and human ABCG2 siRNA.

Techniques: Expressing, Staining, Knockdown, Western Blot, Transfection, Plasmid Preparation, Cell Culture, Incubation

GPX4, CAT, and GSR regulate chemosensitivity through Nrf2-mediated ABCG2 expression. (a and b) qPCR analysis of ABCG2 and NQO1 mRNA expression in paired GBC-SD (a) and QBC-939 (b) cells transfected with or without siRNA of GPX4, GSR, CAT . n = 3; Bar, SEM. (c and d) Immunoblots of ABCG2 and NQO1 in paired GBC-SD (c) and QBC-939 (d) cells that expressed GPX4, GSR , or CAT targeting siRNAs or a scrambled siRNA. (e and f) ABCG2-promoter luciferase assay in paired GBC-SD (a) and QBC-939 (b) cells that were transfected with or without siRNA of GPX4, GSR, CAT . n = 3; Bar, SEM. (g) The canonical sequence of the Nrf2-binding site (top, red), a potential Nrf2-binding site at -431 bp to -420 bp in the proximal promoter region of the human ABCG2 gene (middle, red), and introduced point mutations (bottom, green) used to inactivate the potential ABCG2-binding site are shown. (h and i) Determination of luciferase activity using vector only, wild type or mutant ABCG2 promoter in different pairs of GBC-SD (h) and QBC-939 (i) cells. (j) ChIP analysis of paired GBC-SD (sh-Con and sh- NFE2L2 ) cells immunoprecipitated by anti-Nrf2 or IgG antibody followed by qPCR using 2 primer sets for the Nrf2-binding site in the ABCG2 promoter or ABCG2 exon 1, respectively. Data represent the percent of input. n = 3; Bar, SEM. (k) Doxorubicin efflux of paired GBC-SD (left) and QBC-939 (right) cells were detected by FACS. * P < .05, ** P < .01, *** P < .001, Student's t- test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: EBioMedicine

Article Title: Variants in oxidative stress-related genes affect the chemosensitivity through Nrf2-mediated signaling pathway in biliary tract cancer

doi: 10.1016/j.ebiom.2019.08.037

Figure Lengend Snippet: GPX4, CAT, and GSR regulate chemosensitivity through Nrf2-mediated ABCG2 expression. (a and b) qPCR analysis of ABCG2 and NQO1 mRNA expression in paired GBC-SD (a) and QBC-939 (b) cells transfected with or without siRNA of GPX4, GSR, CAT . n = 3; Bar, SEM. (c and d) Immunoblots of ABCG2 and NQO1 in paired GBC-SD (c) and QBC-939 (d) cells that expressed GPX4, GSR , or CAT targeting siRNAs or a scrambled siRNA. (e and f) ABCG2-promoter luciferase assay in paired GBC-SD (a) and QBC-939 (b) cells that were transfected with or without siRNA of GPX4, GSR, CAT . n = 3; Bar, SEM. (g) The canonical sequence of the Nrf2-binding site (top, red), a potential Nrf2-binding site at -431 bp to -420 bp in the proximal promoter region of the human ABCG2 gene (middle, red), and introduced point mutations (bottom, green) used to inactivate the potential ABCG2-binding site are shown. (h and i) Determination of luciferase activity using vector only, wild type or mutant ABCG2 promoter in different pairs of GBC-SD (h) and QBC-939 (i) cells. (j) ChIP analysis of paired GBC-SD (sh-Con and sh- NFE2L2 ) cells immunoprecipitated by anti-Nrf2 or IgG antibody followed by qPCR using 2 primer sets for the Nrf2-binding site in the ABCG2 promoter or ABCG2 exon 1, respectively. Data represent the percent of input. n = 3; Bar, SEM. (k) Doxorubicin efflux of paired GBC-SD (left) and QBC-939 (right) cells were detected by FACS. * P < .05, ** P < .01, *** P < .001, Student's t- test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The following StableTM siRNA oligo (Genepharma, Shanghai, China) were used: nontargeting scrambled siRNA, human GPX4 siRNA, human CAT siRNA, human GSR siRNA, and human ABCG2 siRNA.

Techniques: Expressing, Transfection, Western Blot, Luciferase, Sequencing, Binding Assay, Activity Assay, Plasmid Preparation, Mutagenesis, Immunoprecipitation

Nrf2 is the key element for growth and chemoresistance of BTC xenografts. (a and c) GBC-SD or QBC-939 cells stably expressing NFE2L2 -targeting shRNA (sh- NFE2L2 ) or scrambled shRNA (sh-Con) were injected into the flank of athymic nude mice s.c. ( n = 6 mice for each group) to create tumor xenografts. After tumors were about 5 mm in diameter, the first paired mice group was treated with saline, the second was treated with cisplatin (6 mg/kg), and the third was combined radical surgery and cisplatin (6 mg/kg) treatment. The chemotherapy was given once every nine days for 1 month. Tumor growth was determined by measurement of tumor volume, tumor weight, and the frequency of tumor formation. Tumor growth curves (left), tumor weigh (middle), and tumor-free percentages (right) at the indicated times were plotted. n = 6; Bar, SEM. (b and d) H&E, TUNEL, and IHC analysis of Ki-67, Nrf2, and ABCG2 expressions in tumor (b: GBC-SD; d: QBC-939) xenografts of the second paired mice groups. Representative images from 6 separate samples are shown. Original magnification, × 400; scale bars: 50 μm. * P < .05, ** P < .01, *** P < .001, Student's t- test.

Journal: EBioMedicine

Article Title: Variants in oxidative stress-related genes affect the chemosensitivity through Nrf2-mediated signaling pathway in biliary tract cancer

doi: 10.1016/j.ebiom.2019.08.037

Figure Lengend Snippet: Nrf2 is the key element for growth and chemoresistance of BTC xenografts. (a and c) GBC-SD or QBC-939 cells stably expressing NFE2L2 -targeting shRNA (sh- NFE2L2 ) or scrambled shRNA (sh-Con) were injected into the flank of athymic nude mice s.c. ( n = 6 mice for each group) to create tumor xenografts. After tumors were about 5 mm in diameter, the first paired mice group was treated with saline, the second was treated with cisplatin (6 mg/kg), and the third was combined radical surgery and cisplatin (6 mg/kg) treatment. The chemotherapy was given once every nine days for 1 month. Tumor growth was determined by measurement of tumor volume, tumor weight, and the frequency of tumor formation. Tumor growth curves (left), tumor weigh (middle), and tumor-free percentages (right) at the indicated times were plotted. n = 6; Bar, SEM. (b and d) H&E, TUNEL, and IHC analysis of Ki-67, Nrf2, and ABCG2 expressions in tumor (b: GBC-SD; d: QBC-939) xenografts of the second paired mice groups. Representative images from 6 separate samples are shown. Original magnification, × 400; scale bars: 50 μm. * P < .05, ** P < .01, *** P < .001, Student's t- test.

Article Snippet: The following StableTM siRNA oligo (Genepharma, Shanghai, China) were used: nontargeting scrambled siRNA, human GPX4 siRNA, human CAT siRNA, human GSR siRNA, and human ABCG2 siRNA.

Techniques: Stable Transfection, Expressing, shRNA, Injection, Saline, TUNEL Assay